h 2b lymphoma t cell line Search Results


el4 lymphoma cells  (ATCC)
98
ATCC el4 lymphoma cells
El4 Lymphoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human bcl-2 (b-cell leukemia/lymphoma 2) elisa kit  (Guangzhou JET Bio-Filtration)
94
Guangzhou JET Bio-Filtration human bcl-2 (b-cell leukemia/lymphoma 2) elisa kit
Human Bcl 2 (B Cell Leukemia/Lymphoma 2) Elisa Kit, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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b-cell lymphoma-2 (bcl-2) (26 kda, rabbit polyclonal antibody)  (Affinity Biosciences)
90
Affinity Biosciences b-cell lymphoma-2 (bcl-2) (26 kda, rabbit polyclonal antibody)
Apoptosis, glutathione (GSH) synthesis, and DNA damage-related protein expression in cholangiocarcinoma cells (CCAs) with co-treatment of babaodan (BBD) and cisplatin. Western blot was used to measure protein levels ( n = 3). BBD decreased (a) the <t>bcl-2</t> level and (b) increased the bax level in a dose-dependent manner. Change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In CCAs involving cisplatin, the expression levels of (d) p-YAP1 relative to total YAP1, (e) SLC1A5, and (f) ATF4, which are involved in GSH synthesis, were observed to decrease in a dose-dependent manner following BBD treatment. (g) the γH2Ax level was increased by 1 mg/mL of BBD treatment, and (h) the ERCC1 level was inhibited by treatment with 0.5 and 1 mg/mL of BBD treatment. Representative protein bands are shown in the (i), (j), and (k). (mean ± Standard deviation) * p < 0.05, ** p < 0.01, vs. control group; # p < 0.05, ## p < 0.01, vs. CDDP group.
B Cell Lymphoma 2 (Bcl 2) (26 Kda, Rabbit Polyclonal Antibody), supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/b-cell lymphoma-2 (bcl-2) (26 kda, rabbit polyclonal antibody)/product/Affinity Biosciences
Average 90 stars, based on 1 article reviews
b-cell lymphoma-2 (bcl-2) (26 kda, rabbit polyclonal antibody) - by Bioz Stars, 2026-02
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bcl-2 #2870 antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc bcl-2 #2870 antibody
Apoptosis, glutathione (GSH) synthesis, and DNA damage-related protein expression in cholangiocarcinoma cells (CCAs) with co-treatment of babaodan (BBD) and cisplatin. Western blot was used to measure protein levels ( n = 3). BBD decreased (a) the <t>bcl-2</t> level and (b) increased the bax level in a dose-dependent manner. Change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In CCAs involving cisplatin, the expression levels of (d) p-YAP1 relative to total YAP1, (e) SLC1A5, and (f) ATF4, which are involved in GSH synthesis, were observed to decrease in a dose-dependent manner following BBD treatment. (g) the γH2Ax level was increased by 1 mg/mL of BBD treatment, and (h) the ERCC1 level was inhibited by treatment with 0.5 and 1 mg/mL of BBD treatment. Representative protein bands are shown in the (i), (j), and (k). (mean ± Standard deviation) * p < 0.05, ** p < 0.01, vs. control group; # p < 0.05, ## p < 0.01, vs. CDDP group.
Bcl 2 #2870 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bcl-2 #2870 antibody/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
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antibody for b-cell lymphoma 2 bcl-2  (Sangon Biotech)
90
Sangon Biotech antibody for b-cell lymphoma 2 bcl-2
Apoptosis, glutathione (GSH) synthesis, and DNA damage-related protein expression in cholangiocarcinoma cells (CCAs) with co-treatment of babaodan (BBD) and cisplatin. Western blot was used to measure protein levels ( n = 3). BBD decreased (a) the <t>bcl-2</t> level and (b) increased the bax level in a dose-dependent manner. Change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In CCAs involving cisplatin, the expression levels of (d) p-YAP1 relative to total YAP1, (e) SLC1A5, and (f) ATF4, which are involved in GSH synthesis, were observed to decrease in a dose-dependent manner following BBD treatment. (g) the γH2Ax level was increased by 1 mg/mL of BBD treatment, and (h) the ERCC1 level was inhibited by treatment with 0.5 and 1 mg/mL of BBD treatment. Representative protein bands are shown in the (i), (j), and (k). (mean ± Standard deviation) * p < 0.05, ** p < 0.01, vs. control group; # p < 0.05, ## p < 0.01, vs. CDDP group.
Antibody For B Cell Lymphoma 2 Bcl 2, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody for b-cell lymphoma 2 bcl-2/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
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b-cell lymphoma 2 antibody  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology b-cell lymphoma 2 antibody
Apoptosis, glutathione (GSH) synthesis, and DNA damage-related protein expression in cholangiocarcinoma cells (CCAs) with co-treatment of babaodan (BBD) and cisplatin. Western blot was used to measure protein levels ( n = 3). BBD decreased (a) the <t>bcl-2</t> level and (b) increased the bax level in a dose-dependent manner. Change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In CCAs involving cisplatin, the expression levels of (d) p-YAP1 relative to total YAP1, (e) SLC1A5, and (f) ATF4, which are involved in GSH synthesis, were observed to decrease in a dose-dependent manner following BBD treatment. (g) the γH2Ax level was increased by 1 mg/mL of BBD treatment, and (h) the ERCC1 level was inhibited by treatment with 0.5 and 1 mg/mL of BBD treatment. Representative protein bands are shown in the (i), (j), and (k). (mean ± Standard deviation) * p < 0.05, ** p < 0.01, vs. control group; # p < 0.05, ## p < 0.01, vs. CDDP group.
B Cell Lymphoma 2 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/b-cell lymphoma 2 antibody/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
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bcl 2  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology bcl 2
Apoptosis, glutathione (GSH) synthesis, and DNA damage-related protein expression in cholangiocarcinoma cells (CCAs) with co-treatment of babaodan (BBD) and cisplatin. Western blot was used to measure protein levels ( n = 3). BBD decreased (a) the <t>bcl-2</t> level and (b) increased the bax level in a dose-dependent manner. Change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In CCAs involving cisplatin, the expression levels of (d) p-YAP1 relative to total YAP1, (e) SLC1A5, and (f) ATF4, which are involved in GSH synthesis, were observed to decrease in a dose-dependent manner following BBD treatment. (g) the γH2Ax level was increased by 1 mg/mL of BBD treatment, and (h) the ERCC1 level was inhibited by treatment with 0.5 and 1 mg/mL of BBD treatment. Representative protein bands are shown in the (i), (j), and (k). (mean ± Standard deviation) * p < 0.05, ** p < 0.01, vs. control group; # p < 0.05, ## p < 0.01, vs. CDDP group.
Bcl 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bcl 2/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
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b cell lymphoma 2  (Danaher Inc)
86
Danaher Inc b cell lymphoma 2
Apoptosis, glutathione (GSH) synthesis, and DNA damage-related protein expression in cholangiocarcinoma cells (CCAs) with co-treatment of babaodan (BBD) and cisplatin. Western blot was used to measure protein levels ( n = 3). BBD decreased (a) the <t>bcl-2</t> level and (b) increased the bax level in a dose-dependent manner. Change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In CCAs involving cisplatin, the expression levels of (d) p-YAP1 relative to total YAP1, (e) SLC1A5, and (f) ATF4, which are involved in GSH synthesis, were observed to decrease in a dose-dependent manner following BBD treatment. (g) the γH2Ax level was increased by 1 mg/mL of BBD treatment, and (h) the ERCC1 level was inhibited by treatment with 0.5 and 1 mg/mL of BBD treatment. Representative protein bands are shown in the (i), (j), and (k). (mean ± Standard deviation) * p < 0.05, ** p < 0.01, vs. control group; # p < 0.05, ## p < 0.01, vs. CDDP group.
B Cell Lymphoma 2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/b cell lymphoma 2/product/Danaher Inc
Average 86 stars, based on 1 article reviews
b cell lymphoma 2 - by Bioz Stars, 2026-02
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b cell lymphoma 2 bcl 2  (Danaher Inc)
86
Danaher Inc b cell lymphoma 2 bcl 2
Anti-IL-9 antibody promoted phagocytosis and inhibited apoptosis of macrophages in MRSA-infected mice. Alveolar macrophages isolated from mice (n = 6 per group) were cocultured with MRSA prestained with FITC, and macrophage phagocytosis was assessed by flow cytometry (A and B) and laser scanning confocal microscopy (C and D), but they did not differentiate surface-bound and internalized bacteria. The protein levels of cleaved CASPASE-3, BAX, and <t>BCL-2</t> and the expression of IL-4, TGF-β, IFN-γ, and IL-17 in the lung tissues of mice were detected by western blot (E and F) and ELISA (G–J), respectively. Data are represented as the mean ± SD. **P < 0.01, ***P < 0.001 vs the MRSA group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs the IgG group.
B Cell Lymphoma 2 Bcl 2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/b cell lymphoma 2 bcl 2/product/Danaher Inc
Average 86 stars, based on 1 article reviews
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antibodies of b-cell lymphoma-2 (bcl-2)  (GeneTex)
90
GeneTex antibodies of b-cell lymphoma-2 (bcl-2)
Anti-IL-9 antibody promoted phagocytosis and inhibited apoptosis of macrophages in MRSA-infected mice. Alveolar macrophages isolated from mice (n = 6 per group) were cocultured with MRSA prestained with FITC, and macrophage phagocytosis was assessed by flow cytometry (A and B) and laser scanning confocal microscopy (C and D), but they did not differentiate surface-bound and internalized bacteria. The protein levels of cleaved CASPASE-3, BAX, and <t>BCL-2</t> and the expression of IL-4, TGF-β, IFN-γ, and IL-17 in the lung tissues of mice were detected by western blot (E and F) and ELISA (G–J), respectively. Data are represented as the mean ± SD. **P < 0.01, ***P < 0.001 vs the MRSA group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs the IgG group.
Antibodies Of B Cell Lymphoma 2 (Bcl 2), supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies of b-cell lymphoma-2 (bcl-2)/product/GeneTex
Average 90 stars, based on 1 article reviews
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rabbit anti-human caspase-9  (Proteintech)
90
Proteintech rabbit anti-human caspase-9
Effects of apatinib on the expression of apoptosis-related genes in SMMC-7721 cells detected by RT-qPCR. Compared with the control group, apatinib significantly increased the expression levels of <t>Bax</t> and caspase-9 mRNA and inhibited the expression <t>of</t> <t>Bcl-2</t> mRNA in SMMC-7721 cells. **P<0.01, compared with control group.
Rabbit Anti Human Caspase 9, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bcl 2  (Proteintech)
96
Proteintech bcl 2
Effects of apatinib on the expression of apoptosis-related genes in SMMC-7721 cells detected by RT-qPCR. Compared with the control group, apatinib significantly increased the expression levels of <t>Bax</t> and caspase-9 mRNA and inhibited the expression <t>of</t> <t>Bcl-2</t> mRNA in SMMC-7721 cells. **P<0.01, compared with control group.
Bcl 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Apoptosis, glutathione (GSH) synthesis, and DNA damage-related protein expression in cholangiocarcinoma cells (CCAs) with co-treatment of babaodan (BBD) and cisplatin. Western blot was used to measure protein levels ( n = 3). BBD decreased (a) the bcl-2 level and (b) increased the bax level in a dose-dependent manner. Change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In CCAs involving cisplatin, the expression levels of (d) p-YAP1 relative to total YAP1, (e) SLC1A5, and (f) ATF4, which are involved in GSH synthesis, were observed to decrease in a dose-dependent manner following BBD treatment. (g) the γH2Ax level was increased by 1 mg/mL of BBD treatment, and (h) the ERCC1 level was inhibited by treatment with 0.5 and 1 mg/mL of BBD treatment. Representative protein bands are shown in the (i), (j), and (k). (mean ± Standard deviation) * p < 0.05, ** p < 0.01, vs. control group; # p < 0.05, ## p < 0.01, vs. CDDP group.

Journal: Pharmaceutical Biology

Article Title: Babaodan overcomes cisplatin resistance in cholangiocarcinoma via inhibiting YAP1

doi: 10.1080/13880209.2024.2331060

Figure Lengend Snippet: Apoptosis, glutathione (GSH) synthesis, and DNA damage-related protein expression in cholangiocarcinoma cells (CCAs) with co-treatment of babaodan (BBD) and cisplatin. Western blot was used to measure protein levels ( n = 3). BBD decreased (a) the bcl-2 level and (b) increased the bax level in a dose-dependent manner. Change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In CCAs involving cisplatin, the expression levels of (d) p-YAP1 relative to total YAP1, (e) SLC1A5, and (f) ATF4, which are involved in GSH synthesis, were observed to decrease in a dose-dependent manner following BBD treatment. (g) the γH2Ax level was increased by 1 mg/mL of BBD treatment, and (h) the ERCC1 level was inhibited by treatment with 0.5 and 1 mg/mL of BBD treatment. Representative protein bands are shown in the (i), (j), and (k). (mean ± Standard deviation) * p < 0.05, ** p < 0.01, vs. control group; # p < 0.05, ## p < 0.01, vs. CDDP group.

Article Snippet: Protein bands were detected to measure the signal expression strength of B-cell lymphoma-2 (Bcl-2) (26 kDa, Rabbit polyclonal antibody) (1:2000; Affinity Biosciences Cat# AF6139, RRID: AB_2835021; USA), Bcl2-associated X (Bax) (21 kDa, Rabbit monoclonal antibody) (1:2000; Abcam Cat# ab182733, RRID: AB_2938987; UK), cleaved-caspase-3 (cle-caspase-3) (17 kDa, Rabbit monoclonal antibody) (1:500; Abcam Cat# ab32042, RRID: AB_725947), caspase-3 (34 kDa, Rabbit polyclonal antibody) (1:500; Abcam Cat# ab13847, RRID: AB_443014), p-YAP1 (54 kDa, Rabbit polyclonal antibody) (1:2000; Affinity Biosciences Cat# AF3328, RRID: AB_2810276), YAP1 (54 kDa, Rabbit polyclonal antibody) (1:2000; Affinity Biosciences Cat# AF6328, RRID: AB_ 2835184), activating transcription factor 4 (ATF4) (39 kDa, Rabbit polyclonal antibody) (1:2000; Affinity Biosciences Cat# DF6008, RRID: AB_2833025), solute carrier family 1 member 5 (SLC1A5) (56 kDa, Rabbit polyclonal antibody) (1:1000; Affinity Biosciences Cat# AF6610, RRID: AB_2843431), gamma histone (γH2Ax) (15 kDa, Rabbit monoclonal antibody) (1:5000; Abcam Cat# ab81299, RRID: AB_1640564), ERCC excision repair 1 (ERCC1) (36 kDa, Rabbit polyclonal antibody) (1:2000; Affinity Biosciences Cat# AF0154, RRID: AB_2833335), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (37 kDa, Rabbit polyclonal antibody) (1:10 000; Affinity Biosciences Cat# AF7021, RRID: AB_2839421) by a gel image processing system (610020-9Q, Clinx, China).

Techniques: Expressing, Western Blot, Standard Deviation, Control

The extent of apoptosis, glutathione (GSH) synthesis, and the expression of DNA damage-related proteins were assessed in cholangiocarcinoma cells (CCAs) subjected to YAP1 knockdown or YAP1 overexpression. Western blot was used to measure protein levels ( n = 3). With cisplatin incubation, YAP1 knockdown and 1 mg/mL babaodan (BBD) treatment decreased the (a) Bcl-2 level and (b) increased bax level, while the change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In the CCAs dealing with cisplatin, the expression levels of (d) p-YAP1/YAP1, (e) ATF4, and (f) SLC1A5 were decreased by YAP1 knockdown and BBD treatment. Additionally, the (g) γH2Ax level was increased and (h) the ERCC1 level was inhibited by YAP1 knockdown and BBD treatment. YAP1 overexpression antagonized the effect of BBD on these proteins. Representative protein bands are shown in (i), (j), and (k). (mean ± standard deviation) + p < 0.05, ++ p < 0.01, vs. CDDP group; # p < 0.05, ## p < 0.01, vs. CDDP + BBD group.

Journal: Pharmaceutical Biology

Article Title: Babaodan overcomes cisplatin resistance in cholangiocarcinoma via inhibiting YAP1

doi: 10.1080/13880209.2024.2331060

Figure Lengend Snippet: The extent of apoptosis, glutathione (GSH) synthesis, and the expression of DNA damage-related proteins were assessed in cholangiocarcinoma cells (CCAs) subjected to YAP1 knockdown or YAP1 overexpression. Western blot was used to measure protein levels ( n = 3). With cisplatin incubation, YAP1 knockdown and 1 mg/mL babaodan (BBD) treatment decreased the (a) Bcl-2 level and (b) increased bax level, while the change in (c) cle-caspase-3/caspase-3 levels with BBD treatment was not statistically significant. In the CCAs dealing with cisplatin, the expression levels of (d) p-YAP1/YAP1, (e) ATF4, and (f) SLC1A5 were decreased by YAP1 knockdown and BBD treatment. Additionally, the (g) γH2Ax level was increased and (h) the ERCC1 level was inhibited by YAP1 knockdown and BBD treatment. YAP1 overexpression antagonized the effect of BBD on these proteins. Representative protein bands are shown in (i), (j), and (k). (mean ± standard deviation) + p < 0.05, ++ p < 0.01, vs. CDDP group; # p < 0.05, ## p < 0.01, vs. CDDP + BBD group.

Article Snippet: Protein bands were detected to measure the signal expression strength of B-cell lymphoma-2 (Bcl-2) (26 kDa, Rabbit polyclonal antibody) (1:2000; Affinity Biosciences Cat# AF6139, RRID: AB_2835021; USA), Bcl2-associated X (Bax) (21 kDa, Rabbit monoclonal antibody) (1:2000; Abcam Cat# ab182733, RRID: AB_2938987; UK), cleaved-caspase-3 (cle-caspase-3) (17 kDa, Rabbit monoclonal antibody) (1:500; Abcam Cat# ab32042, RRID: AB_725947), caspase-3 (34 kDa, Rabbit polyclonal antibody) (1:500; Abcam Cat# ab13847, RRID: AB_443014), p-YAP1 (54 kDa, Rabbit polyclonal antibody) (1:2000; Affinity Biosciences Cat# AF3328, RRID: AB_2810276), YAP1 (54 kDa, Rabbit polyclonal antibody) (1:2000; Affinity Biosciences Cat# AF6328, RRID: AB_ 2835184), activating transcription factor 4 (ATF4) (39 kDa, Rabbit polyclonal antibody) (1:2000; Affinity Biosciences Cat# DF6008, RRID: AB_2833025), solute carrier family 1 member 5 (SLC1A5) (56 kDa, Rabbit polyclonal antibody) (1:1000; Affinity Biosciences Cat# AF6610, RRID: AB_2843431), gamma histone (γH2Ax) (15 kDa, Rabbit monoclonal antibody) (1:5000; Abcam Cat# ab81299, RRID: AB_1640564), ERCC excision repair 1 (ERCC1) (36 kDa, Rabbit polyclonal antibody) (1:2000; Affinity Biosciences Cat# AF0154, RRID: AB_2833335), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (37 kDa, Rabbit polyclonal antibody) (1:10 000; Affinity Biosciences Cat# AF7021, RRID: AB_2839421) by a gel image processing system (610020-9Q, Clinx, China).

Techniques: Expressing, Knockdown, Over Expression, Western Blot, Incubation, Standard Deviation

Anti-IL-9 antibody promoted phagocytosis and inhibited apoptosis of macrophages in MRSA-infected mice. Alveolar macrophages isolated from mice (n = 6 per group) were cocultured with MRSA prestained with FITC, and macrophage phagocytosis was assessed by flow cytometry (A and B) and laser scanning confocal microscopy (C and D), but they did not differentiate surface-bound and internalized bacteria. The protein levels of cleaved CASPASE-3, BAX, and BCL-2 and the expression of IL-4, TGF-β, IFN-γ, and IL-17 in the lung tissues of mice were detected by western blot (E and F) and ELISA (G–J), respectively. Data are represented as the mean ± SD. **P < 0.01, ***P < 0.001 vs the MRSA group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs the IgG group.

Journal: Infection and Immunity

Article Title: IL-9 promotes methicillin-resistant Staphylococcus aureus pneumonia by regulating the polarization and phagocytosis of macrophages

doi: 10.1128/iai.00166-23

Figure Lengend Snippet: Anti-IL-9 antibody promoted phagocytosis and inhibited apoptosis of macrophages in MRSA-infected mice. Alveolar macrophages isolated from mice (n = 6 per group) were cocultured with MRSA prestained with FITC, and macrophage phagocytosis was assessed by flow cytometry (A and B) and laser scanning confocal microscopy (C and D), but they did not differentiate surface-bound and internalized bacteria. The protein levels of cleaved CASPASE-3, BAX, and BCL-2 and the expression of IL-4, TGF-β, IFN-γ, and IL-17 in the lung tissues of mice were detected by western blot (E and F) and ELISA (G–J), respectively. Data are represented as the mean ± SD. **P < 0.01, ***P < 0.001 vs the MRSA group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs the IgG group.

Article Snippet: Equal amounts of proteins were resolved by SDS-PAGE, transferred onto a nitrocellulose membrane and incubated with primary antibodies specific for cleaved cysteine protease 3 (CASPASE-3) (ab214430, 1:5,000; Abcam, USA), BCL2-Associated X (BAX) (ab32503, 1:1,000; Abcam, USA), B-cell lymphoma 2 (BCL-2) (ab182858, 1:2,000; Abcam, USA), PI3K (ab191606, 1:1,000; Abcam, USA), p-PI3K (ab182651, 1:1,000; Abcam, USA), AKT (sc-5298, 1:500; Santa Cruz Biotechnology, CA), p-AKT (sc-514032, 1:500; Santa Cruz Biotechnology, CA), mTOR (sc-517464, 1:1,000; Santa Cruz Biotechnology, CA), and GAPDH (sc-47724, 1:1,000, Santa Cruz Biotechnology).

Techniques: Infection, Isolation, Flow Cytometry, Confocal Microscopy, Bacteria, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Il9 deletion promoted phagocytosis and inhibited apoptosis in MRSA-infected RAW264.7 cells. RAW264.7 cells were infected with MRSA, and Il9 siRNA was employed to delete Il9 in RAW264.7 cells. Flow cytometry was performed to assess the phagocytosis ability (A and B) and apoptosis (C and D) of RAW264.7 cells. (E and F) The protein levels of cleaved CASPASE-3, BAX, and BCL-2 in RAW264.7 cells were detected by western blot. Data are represented as the mean ± SD. n = 3. ***P < 0.001 vs the MRSA group; ### P < 0.001 vs the Si-NC group.

Journal: Infection and Immunity

Article Title: IL-9 promotes methicillin-resistant Staphylococcus aureus pneumonia by regulating the polarization and phagocytosis of macrophages

doi: 10.1128/iai.00166-23

Figure Lengend Snippet: Il9 deletion promoted phagocytosis and inhibited apoptosis in MRSA-infected RAW264.7 cells. RAW264.7 cells were infected with MRSA, and Il9 siRNA was employed to delete Il9 in RAW264.7 cells. Flow cytometry was performed to assess the phagocytosis ability (A and B) and apoptosis (C and D) of RAW264.7 cells. (E and F) The protein levels of cleaved CASPASE-3, BAX, and BCL-2 in RAW264.7 cells were detected by western blot. Data are represented as the mean ± SD. n = 3. ***P < 0.001 vs the MRSA group; ### P < 0.001 vs the Si-NC group.

Article Snippet: Equal amounts of proteins were resolved by SDS-PAGE, transferred onto a nitrocellulose membrane and incubated with primary antibodies specific for cleaved cysteine protease 3 (CASPASE-3) (ab214430, 1:5,000; Abcam, USA), BCL2-Associated X (BAX) (ab32503, 1:1,000; Abcam, USA), B-cell lymphoma 2 (BCL-2) (ab182858, 1:2,000; Abcam, USA), PI3K (ab191606, 1:1,000; Abcam, USA), p-PI3K (ab182651, 1:1,000; Abcam, USA), AKT (sc-5298, 1:500; Santa Cruz Biotechnology, CA), p-AKT (sc-514032, 1:500; Santa Cruz Biotechnology, CA), mTOR (sc-517464, 1:1,000; Santa Cruz Biotechnology, CA), and GAPDH (sc-47724, 1:1,000, Santa Cruz Biotechnology).

Techniques: Infection, Flow Cytometry, Western Blot

Effects of apatinib on the expression of apoptosis-related genes in SMMC-7721 cells detected by RT-qPCR. Compared with the control group, apatinib significantly increased the expression levels of Bax and caspase-9 mRNA and inhibited the expression of Bcl-2 mRNA in SMMC-7721 cells. **P<0.01, compared with control group.

Journal: Oncology Letters

Article Title: Apatinib promotes apoptosis of the SMMC-7721 hepatocellular carcinoma cell line via the PI3K/Akt pathway

doi: 10.3892/ol.2018.8031

Figure Lengend Snippet: Effects of apatinib on the expression of apoptosis-related genes in SMMC-7721 cells detected by RT-qPCR. Compared with the control group, apatinib significantly increased the expression levels of Bax and caspase-9 mRNA and inhibited the expression of Bcl-2 mRNA in SMMC-7721 cells. **P<0.01, compared with control group.

Article Snippet: Materials used in the present study were: Apatinib and DMSO (Aladdin, Shanghai, China); SMMC-7721 hepatocellular carcinoma cell line (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences, Shanghai, China); RPMI-1640 medium (Gibco Life Technologies, Carlsbad, CA, USA); MTT (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany); Annexin V/PI apoptosis detection kit (Beyotime Institute of Biotechnology, Nantong, China); TRIzol, reverse transcription kit and RT-qPCR kit (all from Invitrogen, Carlsbad, CA, USA); primer synthesis (Takara Biotechnology Co., Ltd., Dalian, China); rabbit anti-human PIAK, rabbit anti-human pPI3K, rabbit anti-human Akt, rabbit anti-human pAkt, rabbit anti-human Bcl-2, rabbit anti-human Bax, rabbit anti-human caspase-9, and rabbit anti-human GAPDH primary antibodies, and HRP-labeled goat anti-rabbit secondary antibody (all from Proteintech Group, Inc., Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR

Effects of apatinib on the expression of apoptosis-related proteins in SMMC-7721 cells detected by western blot analysis. (A) Results of western blot analysis. (B) Relative expression levels of each protein. Compared with the control group, apatinib significantly increased the expression levels of Bax and caspase-9 protein and inhibited the expression of Bcl-2 protein in SMMC-7721 cells. **P<0.01, compared with the control group.

Journal: Oncology Letters

Article Title: Apatinib promotes apoptosis of the SMMC-7721 hepatocellular carcinoma cell line via the PI3K/Akt pathway

doi: 10.3892/ol.2018.8031

Figure Lengend Snippet: Effects of apatinib on the expression of apoptosis-related proteins in SMMC-7721 cells detected by western blot analysis. (A) Results of western blot analysis. (B) Relative expression levels of each protein. Compared with the control group, apatinib significantly increased the expression levels of Bax and caspase-9 protein and inhibited the expression of Bcl-2 protein in SMMC-7721 cells. **P<0.01, compared with the control group.

Article Snippet: Materials used in the present study were: Apatinib and DMSO (Aladdin, Shanghai, China); SMMC-7721 hepatocellular carcinoma cell line (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences, Shanghai, China); RPMI-1640 medium (Gibco Life Technologies, Carlsbad, CA, USA); MTT (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany); Annexin V/PI apoptosis detection kit (Beyotime Institute of Biotechnology, Nantong, China); TRIzol, reverse transcription kit and RT-qPCR kit (all from Invitrogen, Carlsbad, CA, USA); primer synthesis (Takara Biotechnology Co., Ltd., Dalian, China); rabbit anti-human PIAK, rabbit anti-human pPI3K, rabbit anti-human Akt, rabbit anti-human pAkt, rabbit anti-human Bcl-2, rabbit anti-human Bax, rabbit anti-human caspase-9, and rabbit anti-human GAPDH primary antibodies, and HRP-labeled goat anti-rabbit secondary antibody (all from Proteintech Group, Inc., Wuhan, China).

Techniques: Expressing, Western Blot